Douglas B. Craig
Biochemistry
Professor
B.Sc.(1988) Mount Allison; Ph.D.(1993) Dalhousie; Post-Doc Manitoba (1993-1994), Alberta (1994-1997)phone: (204) 786-9731
fax: (204) 775-2114
email: d.craig@uwinnipeg.ca
Research Interests
Chemical studies typically involve measurements of large ensembles of
molecules with data representing averages. With respect to enzymes, we now
know such average values represent a simplification. Single enzyme
molecule assay utilizing ultrasensivitive capillary electrophoresis
laser-induced fluorescence instrumentation have demonstrated that
individual enzyme molecules are not identical. Individual molecules differ
with respect to rate and activation energy of catalysis. My research
interests are in the study of the basis and function of the heterogeneity
of enzyme molecules and its role within the cell. A second interest is the
development of ultrasensitive methods for the
analysis of biological molecules, particulary enzymes and proteins.
Image of one of the three capillary electrophoresis instruments in my
laboratory.
So what is
capillary electrophoresis anyhow?
Research Funding
Why would you want
to use a separation method such as CE to assay enzymes?
What's the deal
with single enzyme molecule assays?
Why would you want
to study enzymes one molecule at a time?
Seminars
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Last updated in July 2008.